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Learn practical standards and methodologies for establishing, maintaining, and utilizing patient-derived xenograft (PDX) models in cancer research through this 26-minute webinar presented by Dr. Nhu-An Pham from the Princess Margaret Living Biobank. Explore how PDX models serve as patient tumor avatars that recapitulate phenotype-genotype associations while providing unlimited tissue availability for investigating molecular mechanisms of drug response, tolerance, and resistance. Discover key steps in PDX workflows including fresh tumor specimen collection, engraftment in immune-deficient murine hosts, model propagation, cryobanking, and curation processes. Examine variations in PDX methodologies and gain insights into implementing standard operating procedures that ensure high-quality, reproducible results while capturing both biological and methodological variations. Understand the multi-dimensional nature of PDX data encompassing matched patient tumor clinico-pathological features, molecular profiles, PDX growth characteristics, mouse health parameters, and assay-specific results. Review supporting infrastructures, databases, and computational tools that contribute to standardized cumulative data collection for high-throughput visualization and assessment across models, projects, and laboratories. Master good practices that reduce cost and time burdens while optimizing tumor model quality, experimental design effectiveness, and data shareability for clinical translation in precision cancer medicine.
