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Learn about cutting-edge analytical approaches for characterizing large RNA therapeutics in this 35-minute webinar presented by Dr. Balasubrahmanyam Addepalli, Director of Evaluations and Application Science at Waters Corporation. Explore the development of novel enzyme specificities designed to address the analytical challenges posed by mRNA, sgRNA, and associated impurities in therapeutic applications. Discover how complementary cleavage specificities can fill sequence coverage gaps left by traditional RNases, and understand the implementation of tunable dinucleotide specific enzymes that generate both shorter and longer digestion products. Examine streamlined informatics workflows that enable accurate sequence confirmation with high efficiency and confidence, allowing for comprehensive integrity assessment in a single LC-MS run. Gain insights into the evolution of analytical technologies needed to support the advancement of large RNA therapeutics, including practical applications for mRNA and guide RNA characterization. The presentation addresses the inadequacies of current enzyme and data analysis workflows while proposing innovative solutions for rapid and sensitive characterization of these complex therapeutic molecules. PACE credits are available for registered participants through February 19, 2027.
