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Explore digital PCR technology and its application in liquid biopsy analysis through this 30-minute webinar presented by Christian Kis and Chris Monjure. Learn how digital PCR (dPCR) enables highly sensitive detection of rare targets in liquid biopsies, particularly for identifying and tracking cancer-causing mutations in circulating tumor DNA. Discover the fundamental differences between dPCR and qPCR, and understand why dPCR's high precision and sensitivity make it ideal for detecting low-abundant tumor nucleic acids that represent only a small fraction of total circulating cell-free nucleic acids in serum samples. Examine how dPCR can serve as a simple and precise method for tracking potential treatment response and resistance, and gain practical insights into implementing dPCR technology in laboratory settings. Review the QuantStudio Absolute Q Digital PCR System, including its workflow and software capabilities, along with various nucleic acid extraction options for liquid biopsy samples including ctDNA and cfDNA. Understand how liquid biopsy analysis is increasingly used in research for measuring therapeutic response, quantifying residual tumor burden, and studying resistance to targeted therapies, with particular focus on the challenges of detecting single nucleotide polymorphisms in low abundance amongst wild type background DNA.
