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Learn essential techniques for advanced cellular imaging in this 26-minute lecture covering multidimensional microscopy approaches. Master the process of capturing complex cell images across multiple dimensions including spatial coordinates (x, y, z), time sequences, multiple color channels, and various sample positions. Explore the practical application of Nyquist sampling theorem to achieve optimal image quality while avoiding over and under sampling issues. Discover methods for creating detailed 3D reconstructions from captured data, and gain hands-on knowledge of live cell imaging protocols and time-lapse microscopy techniques for studying dynamic cellular processes.
Syllabus
Lecture 05_6D imaging, live cell imaging, time lapse imaging
Taught by
NPTEL-NOC IITM