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This lecture from the Santa Fe Institute explores the application of landscape and flux theory to understand nonequilibrium biological systems. Join Jin Wang from Stony Brook University as he discusses how physical approaches for systems out of thermodynamic equilibrium can be applied to living organisms. Discover how recent advances in microscopy, single-cell dynamics, and high-throughput data acquisition have provided unprecedented insights into cell dynamics, genetic regulation, and organismal development. Learn about the principles underlying cellular regulatory networks, differentiation, development, cancer, neural network dynamics, population dynamics, ecology, and evolution. The presentation includes examples of cell fate decision making using both low and high throughput experimental data, demonstrating how landscape and flux theory helps build our understanding of life's complex processes.