Coursera Spring Sale
40% Off Coursera Plus Annual!
Grab it
Explore cutting-edge gene editing technology through this keynote webinar presented by Dr. Chase L. Beisel, focusing on a novel approach to precision genome editing using targeted DNA ADP-ribosylation. Discover how the bacterial DNA ADP-ribosyl transferase DarT2, originally part of an anti-phage toxin-antitoxin system, can be repurposed for site-specific DNA modification by appending bulky ADP-ribosyl moieties to thymine in single-stranded DNA. Learn about the innovative fusion of attenuated DarT2 with Cas9 nickase to program site-specific ADP-ribosylation, which produces remarkably different editing outcomes depending on the biological system. Understand how this approach drives efficient homologous recombination with repair templates in bacteria, enabling flexible and scar-free genome editing without base replacement or counterselection, while in eukaryotic systems including yeast, plants, and human cells, it preferentially replaces modified thymine with a bias towards adenine or cytosine with minimal insertions or deletions. Gain insights into Dr. Beisel's research background spanning RNA engineering, CRISPR-Cas systems, and infectious disease applications, drawing from his extensive experience at institutions including Caltech, NIH, NC State University, and the Helmholtz Institute for RNA-based Infection Research. Participate in the live Q&A session to deepen your understanding of this groundbreaking gene editing modality that expands beyond traditional base deamination and removal approaches, offering new possibilities for precision genetic modifications across different domains of life.
