Optimizing Non-viral CRISPR HDR for High-efficiency Large Knock-in in Primary T Cells and iPSCs

Learn advanced CRISPR genome editing techniques in this 23-minute video focused on optimizing homology-directed repair (HDR) for large knock-ins in primary T cells and iPSCs. Explore critical research findings on achieving efficient chimeric antigen receptor (CAR) insertions in primary T lymphocytes through detailed examination of double strand break repair mechanisms, CRISPR reagent selection, and cell handling protocols. Gain insights into using Aldevron Nanoplasmids™ as effective HDR donor templates, with demonstrated success in both primary T cells and induced pluripotent stem cells.