HbA1c Assay Methodologies and Proficiency Testing - Ensuring Accuracy in Diabetes Management

Learn about the critical role of hemoglobin A1c (HbA1c) testing in diabetes management through this comprehensive 36-minute webinar presented by Dr. Vishnu Samara, Assistant Professor of Pathology at the University of Chicago School of Medicine. Explore the fundamental principles behind HbA1c as a stable glycated hemoglobin that reflects average plasma glucose levels over 2-3 months, making it the gold standard for glycemic control assessment regardless of fasting status or time of day. Discover the American Diabetes Association guidelines that define prediabetes (5.7-6.4% HbA1c) and diabetes (≥6.5% HbA1c) through NGSP-certified, DCCT-standardized assays. Examine various HbA1c testing methodologies including ion-exchange high-performance liquid chromatography (HPLC), boronate affinity HPLC, capillary electrophoresis, immunoassay, and enzymatic assays, understanding how each method's principles affect accuracy and reliability. Analyze the analytical and biological limitations of these assays, particularly how hemoglobinopathies and hemoglobin variants can interfere with results depending on the methodology used. Gain insights into CAP proficiency testing limits, CLIA regulations, and the National Glycohemoglobin Standardization Program's ongoing efforts to improve HbA1c testing standards. Understand the clinical significance of accurate HbA1c measurement for optimal patient care management in hyperglycemia and diabetes treatment. Earn PACE credits by completing the webinar and following the certification process through the Labroots platform.