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Explore the intricate world of cellular transport mechanisms in this guest lecture by Mathias Winterhalter. Delve into the complexities of compartmentation and access control in living organisms, with a focus on Gram-negative bacteria and their sophisticated double wall protection. Discover the challenges cells face in harvesting nutrients while rejecting toxic substances. Learn about cutting-edge research aimed at understanding the rate-limiting steps in antibiotic uptake and transport-related resistance. Examine innovative techniques developed to quantify transport across nanometer-thick membranes and membrane channels. Gain insights into ongoing research at CY Cergy Paris Université, where efforts are underway to develop sensors based on solid-state miniaturized chips functionalized with membrane channels. Explore the potential of these hybrid materials, which combine the robustness of solid-state materials with the versatility of channel-forming proteins, and their implications for future applications in cellular biology and medicine.