End-to-End Tools and Services for Interrogation of CRISPR-Cas Associated Genotoxicity

Explore comprehensive tools and services for identifying and characterizing genotoxicity associated with CRISPR-Cas gene editing systems in this 46-minute webinar presented by Ashley Jacobi, Director of Applications and Market Development at IDT. Learn about the critical importance of detecting low-frequency genomic alterations resulting from off-target editing, gRNA synthesis errors, cross-contamination, and other unintended gRNA activities that could lead to genotoxic effects in gene editing therapeutics. Discover an empirical in vivo tag-based off-target nomination service utilizing RNaseH2-dependent amplification technology to generate comprehensive off-target lists for target gRNAs. Understand how rhAmpSeq technology can identify and classify indels and chromosomal rearrangements at on- and off-target loci with superior effectiveness compared to existing methods. Master a sensitive method for detecting gRNA cross-contamination at levels below 0.1% by leveraging unique NHEJ DNA repair fingerprint information. Examine how manufacturing processes can mitigate unintended genomic alterations and explore new tooling and services designed to improve genotoxicity characterization in gene therapy workflows. Gain insights into the assembly of necessary components and expertise for genotoxicity characterization studies, addressing the expense and labor-intensive nature of these critical assessments. Earn PACE credits by completing the webinar and following the provided certification process through Labroots.