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CRISPR-based Microfluidic Biosensing Systems for Molecular Diagnostics

Labroots via YouTube

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Overview

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Explore a 34-minute webinar on CRISPR-based microfluidic biosensing systems for molecular diagnostics presented by Dr. Changchun Liu, associate professor at the University of Connecticut. Delve into the integration of CRISPR and microfluidic technologies for detecting pathogens and their potential in point-of-care diagnostics. Learn about self-contained microfluidic diagnostic systems, electricity-free heating methods, and analytical performance in SARS-CoV-2 detection. Discover optimizations in CRISPR reaction buffers, enhanced NEXT CRISPR detection techniques, and CRISPR-based electrochemical biosensing. Gain insights into the latest advancements in nucleic acid-based molecular detection for early diagnosis of infectious diseases and cancer screening. Earn PACE credits by watching the webinar and following the provided instructions.

Syllabus

Intro
Outline
Microfluidic Technology
Point of Care Diagnostic Technology
Different Detection Methods of Pathogens
Nucleic Acid-based Molecular Detection
CRISPR-based Biosensing and its Challenges
Operation of Self-contained Microfluidic Diagnostic System
Electricity-Free Heating System powered by Hand Warmer
Analytical Performance of Self-contained Microfluidic System
SARS-CoV-2 Detection in Clinical Samples
Optimization of CRISPR Reaction Buffer Systems
Enhanced NEXT CRISPR Detection with 5' end-extended crRNA
Sensitivity Evaluation of NEXT CRISPR Biosensor
Clinical Validation of NEXT CRISPR Biosensor
CRISPR-based Electrochemical Biosensing
EFE Electrochemical CRISPR Biosensor
Fluorescence Detection of EFE CRISPR Biosensor
Effect of the Frequency on the CRISPR-based Biosensor
Effect of Peak-to-peak Amplitude on CRISPR-based Biosensor
Optimization of Electroactive ss-DNA Methylene Blue (MB) Probe
Conclusion

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