Cell Culture Basics

Learn essential cell culture techniques through this comprehensive 20-minute video training series designed for safe laboratory practices. Master fundamental aseptic techniques including proper use of 70% ethanol and personal protective equipment, sterile manipulation of culture media bottles, and contamination prevention methods for lids and caps. Develop skills in adding and removing sterile liquids from containers, proper labeling techniques for culture vessels and flasks, and precise liquid handling for both large and small volumes. Practice critical cell maintenance procedures such as aspirating and adding medium to culture dishes and flasks with attached cells, managing culture vessels in incubators, and seeding cells into various culture containers. Complete your training by learning proper cleanup protocols for aspirating equipment and biohazardous waste disposal, ensuring laboratory safety for all users and maintaining optimal conditions for cultured cells throughout freezing, thawing, and passaging processes.

Syllabus

Cell Culture Basics 01: Use of 70 % Ethanol and PPE
Cell Culture Basics 02: Preparing to use the bottle of medium
Cell Culture Basics 03: Manipulating lids and caps to reduce the risk of contamination
Cell Culture Basics 04: Adding and removing sterile liquid from a container
Cell Culture Basics 05: Labeling culture vessels with a lid
Cell Culture Basics 06: Labeling cell culture flasks
Cell Culture Basics 07: Adding a liquid to a flask and a series of flasks
Cell Culture Basics 08: Aspirating medium from a culture dish with attached cells
Cell Culture Basics 09: Adding medium to a culture dish with attached cells
Cell Culture Basics 10: Aspirating medium from a culture flask with attached cells
Cell Culture Basics 11: Adding medium to a culture flask with attached cells
Cell Culture Basics 12: Taking tiny volumes of liquids
Cell Culture Basics 13: Bringing and keeping culture vessels inside the cell culture incubator
Cell Culture Basics 14: Adding cells to a cell culture dish
Cell Culture Basics 15: Adding cells to a cell culture flask
Cell Culture Basics 16: Cleaning the aspirating tube once done with cell culture