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YouTube

Cell and Tissue Culture - Primary Cell Lines, Plant and Animal Culture, and Clinical Applications

Animated biology With arpan via YouTube

Overview

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Learn the fundamentals of cell and tissue culture through this comprehensive video series covering both plant and animal cell culture techniques. Explore primary cell culture methods, cell line development, and the differences between cell lines and cell strains. Master essential laboratory procedures including sub-culturing techniques, media preparation for animal cell cultures, and microcarrier culture systems used in industrial applications. Discover advanced biotechnology applications such as hybridoma technology for monoclonal antibody production and stem cell properties, metabolism, and clinical applications. Examine apoptosis detection methods using Annexin-PI assays and understand the practical applications of both 2D and 3D cell culture systems in clinical, industrial, and research settings. Gain insights into plant tissue culture overview and understand the industrial importance and advantages of various culture techniques used in modern biotechnology and pharmaceutical industries.

Syllabus

Primary Cell culture and cell line | Cell culture basics
Plant tissue culture overview |
Animal cell culture media
Sub-culturing cells || Cell culture basics
Hybridoma technology | Monoclonal antibody production using hybridoma technology | Animated biology
Stem cells | properties, metabolism and clinical usage
Microcarrier culture || Industrial importance || Application and advantages
Annexin-PI Assay For Apoptosis | Annexin 5 Assay | Annexin V for early stage apoptosis detection
Application of 2D and 3D cell culture systems | Clinical, Industrial and Research applications
Cell line and cell strain | What is cell strain? | Applications of cell line | Animated biology

Taught by

Animated biology With arpan

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