Coursera Spring Sale
40% Off Coursera Plus Annual!
Grab it
Explore isothermal amplification techniques that streamline nucleic acid amplification without thermal cycling in this comprehensive webinar presented by Raminta Mineikaitė and Rasa Sukackaitė, PhD. Discover two innovative methods - Recombinase Polymerase Amplification (RPA) and Rolling Circle Amplification (RCA) - and learn how they enhance downstream analysis and therapeutic applications. Master RPA, which operates at constant low temperatures (37-45°C) using only two specific primers, providing a rapid and sensitive alternative to traditional PCR methods. See practical demonstrations of RPA reactions as low-temperature NGS library amplification options. Understand RCA's capability to amplify circular DNA templates from minimal starting material, addressing common challenges like low yield and contamination from host organisms used in plasmid purification. Learn how RCA streamlines workflows, significantly reducing experimental time for protein expression and mRNA production. Examine robust RCA-based methods for SNP detection, making it valuable for genetic research and clinical diagnostics. Gain insights into practical applications that can transform your laboratory workflows and improve efficiency in molecular biology research. The webinar offers PACE credits for continuing education and provides actionable knowledge for researchers working in genomics, molecular diagnostics, and biotechnology applications.
