Achieving High Productivities through Bioreactor Optimization at Lower than Peak Cell Densities in a Perfusion Process

Learn how to optimize perfusion bioreactor processes to achieve high productivities without reaching peak cell densities in this 47-minute webinar presented by Dr. Luis Rodriguez, Bioproduction R&D Manager at FUJIFILM Irvine Scientific. Explore the challenges of maintaining highly productive cells over extended periods in high cell density bioreactors and discover cost-effective solutions using small-scale optimization models. Examine the evaluation of BalanCD CHO Perfusion and Perfusion Media Survey Panel through perfusion mimic models and table-top perfusion-capable bioreactors featuring alternating tangential flow filtration units. Understand the scaling process from small-scale perfusion mimic models to production bioreactors in both N-1 perfusion processes and recombinant antibody production. Analyze the relationship between productivity and various metabolites, glucose levels, and aeration parameters during continuous steady-state culture operations. Gain insights into achieving successful high productivities in continuous steady-state perfusion cultures through strategic bioreactor parameter optimization while utilizing appropriate cell culture media, demonstrating that peak cell densities are not always necessary for optimal therapeutic recombinant protein production.