Accelerating Monkeypox and SARS-CoV-2 Virus Whole Genome Sequencing Using Combination of NextGenPCR and Oxford Nanopore - Towards Real-Time Genomic Surveillance

Explore cutting-edge techniques for accelerating whole genome sequencing of Monkeypox and SARS-CoV-2 viruses in this informative 18-minute webinar. Delve into the combination of NextGenPCR and Oxford Nanopore technologies to achieve real-time genomic surveillance. Learn how rapid genomic surveillance can guide public health interventions and discover novel workflows using nanopore technology for real-time sequencing of clinical samples. Understand the limitations of current library preparation workflows and how NextGenPCR technology can significantly reduce PCR library preparation time. Gain insights into the development and optimization of an amplicon-based sequencing approach for Monkeypox, inspired by the SARS-CoV-2 Midnight protocol and ARTIC network. Discover how this innovative workflow allows for increased coverage depth and multiplexing of up to 20 samples per flowcell, making sequencing more cost-effective. Explore a real-world application of this technology in tracking the spread of Monkeypox in Amsterdam, including its role in identifying the first pediatric case in the Netherlands.