Accelerating Insights into Changes in Binding Affinity of Biomolecular Charge Variants Using icIEF Fractionation and SPR Workflow

Join a 45-minute webinar led by Dr. Michael Piazza and Dr. Chris Heger exploring an innovative workflow for analyzing charge variants in monoclonal antibodies. Learn about a fast and efficient method combining icIEF fractionation and digital SPR to characterize therapeutic bispecific antibodies. Discover how to implement a simplified workflow using imaged capillary isoelectric focusing-based charge separation followed by surface plasmon resonance analysis. Examine detailed case studies comparing Mosunetuzumab and a research-grade biosimilar, revealing critical differences in binding affinity with CD3 and CD20 ligands. Gain insights into how mass spectrometry analysis provides additional context for understanding charge variant differences. Master techniques for evaluating product quality and ensuring thorough characterization of biotherapeutic molecules using benchtop platforms.