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Join a 43-minute webinar featuring experts Dr. Ke Ma and Ashleigh Koontz, M.S. from Thermo Fisher Scientific as they explore the analysis of adeno-associated viruses (AAVs) using advanced size exclusion chromatography techniques. Learn about the critical role of SEC in characterizing AAV vectors for gene therapy applications, with particular focus on separating monomeric capsids from high molecular weight species using a specialized SEC column packed with 550 Å pore size, 3μm monodisperse silica particles. Discover how monodisperse particles with proprietary diol hydrophilic coating improve column-to-column reproducibility and minimize undesired interactions. Master key concepts including the significance of SEC in AAV characterization, advantages of monodisperse silica particles, effects of pore size on separation performance, and crucial operational parameters like flow rate. Gain valuable insights into quality control methods essential for AAV-based gene therapy development, including the integration of SEC with complementary techniques such as ELISA, cryo-EM, and AUC.
