In recent years, with the rapid development of the scale, intensification and modernization of China's aquaculture industry, the pressure of livestock and poultry disease infection is increasing, and the complexity of epidemic diseases is increasing. At present, it is difficult for veterinarians to correctly grasp and diagnose based on clinical experience and pathological changes, and testing with the help of professional laboratories is one of the effective ways to diagnose epidemic diseases. Laboratory testing of animal diseases is an important basis for the prevention and control of animal diseases, and is the basic means to evaluate the effect of compulsory immunization and prevention and control of major animal diseases. Through the study of this course, students will understand the basic knowledge of animal disease testing, testing data processing, testing results application and other professional knowledge. Master the professional skills of animal disease detection and sampling technology, animal disease laboratory testing methods, animal disease purification, etc.; Cultivate students' awareness of laboratory biosafety and protection, and establish a new concept of modern pasture animal health care and disease prevention and control.

Syllabus

Chapter 1: Entering the Animal Disease Testing Laboratory

1.1 The importance of animal disease detection technology in animal husbandry
1.2.1 Laboratory design layout
1.2.2 Laboratory management procedures
1.2.3 Biosafety and protection in laboratories
1.3.1 Use and maintenance of commonly used laboratory instruments (1)
1.3.2 Use and maintenance of commonly used laboratory instruments (2)
1.4 Overview of animal disease testing methods

Chapter 2: Preparation of test samples

2.1.1 Methods of blood collection for poultry
2.1.2 Methods of blood collection from pigs
2.1.3 Blood collection methods for cattle and sheep
2.1.4 Blood collection methods for experimental animals
2.2 Preparation of blood samples

Chapter 3: Pathogenicity Testing Techniques

3.1.1 Overview of laboratory diagnosis of bacterial infectious diseases
3.1.2 Use and maintenance of microscope oil lenses
3.1.3 Morphological examination of bacteria
3.1.4 Preparation of commonly used culture media
3.1.5 Isolation and culture techniques of bacteria（一）
3.1.6 Isolation and culture techniques of bacteria（二）
3.2. Laboratory diagnosis of viral infectious diseases

Chapter 4: Serological Testing Techniques

4.1 Overview of serological testing techniques
4.2.1 Overview of agglutination tests
4.2.2 Agglutination test of chicken dysentery plate
4.2.3 Brucellosis test-tube agglutination test
4.2.4 Newcastle disease hemagglutination test and hemagglutination inhibition test (1)
4.2.5 Newcastle disease hemagglutination test and hemagglutination inhibition test (2)
4.3.1 Overview of precipitation tests
4.3.2 Ring precipitation test for anthrax disease
4.3.3 Avian influenza agar diffusion test
4.4.1 Overview of complement fixation testing
4.4.2 Porcine typhoid complement fixation test
4.5.1 Overview of neutralization tests
4.5.2 Porcine pseudorabies neutralization test
4.6.1 Overview of immunolabeling techniques
4.6.2 Swine fever fluorescent antibody staining test
4.6.3 Rapid detection of colloidal gold for canine parvovirus disease
4.6.4 Rapid colloidal gold test for canine microvirus disease