NMR Spectroscopy for Structural Biology

ABOUT THE COURSE:This course will start with Basic principles of NMR, Discuss practical aspects of NMR spectroscopy, peptide NMR, Protein NMR, Nucleic acids NMR. We will teach with examples how to solve structure and measure dynamics using NMR.PRE-REQUISITES: Under graduate level understanding of Physics and Mathematics, Basic course on NMR spectroscopy.INTENDED AUDIENCE: M.Sc./ PhD students & Scientist, and Scientists working in Biopharma Industries.INDUSTRY SUPPORT: Biocon, Sunpharma, Alembic, Bharat Biotech, Aurobindo Biopharma etc.

Syllabus

Week 1:Basics Principles of NMR; Nuclear Spin in static magnetic field Week 2:Basics Principles continued; Chemical Shift; Factors Influencing Isotropic Chemical shifts Week 3:NMR instrumentation and Peptide sample preparation; Overview of NMR instrument Magnet, transmitters etc Week 4:Two-dimensional NMR And Practical Aspects for experiment; Two- dimensional correlation experiments COSY Week 5:Peptide and Protein structure Structural features and parameters; Structural features and parameters Week 6:Sample preparation for Protein NMR; Expression systems, Optimization of protein expression Week 7:Multi-dimensional Heteronuclear NMR experiments; 2D Heteronuclea r NMR experiments Week 8:Multi-dimensional Heteronuclear NMR experiments; Experiments for Backbone assignment
Week 9:Protein Structure determination from NMR data; Structure calculation methods and NMR parameters
Week 10:Protein dynamics from NMR data; Theory of spin relaxation in proteins Week 11:Nucleic acids NMR experiments; Structural features and parameters for Nuclei acids Week 12:DNA and structure determination from NMR data