ABOUT THE COURSE: In the current MOOCs course I have put effort to discuss different aspects of enzymology for engineer graduate students as well as MSc students. Enzyme play pivotal role in running different types of metabolic reactions, converting toxic substances into non-toxic products and they are essential for running several physiological processes.1. Basics understanding of enzyme structure and functions, its production through different types strategies, purification etc. 2. Understanding different approaches to study the interaction of substrate with enzymes, enzyme assay and kinetics. 3. Broad over-view of different approaches to design inhibitors against enzyme, understanding the enzyme-inhibitor kinetics and mode inhibition. 4. Role of enzyme in cell metabolism, physiology and application of enzymes.INTENDED AUDIENCE: UG/PGPREREQUISITES: Basic BiochemistryINDUSTRY SUPPORT: Biocon https://www.biocon.com/Jubilant Life Sciences www.jubl.com/Shantha Biotechnics LtdPanacea Biotec Dr. Reddy’s laboratories

Syllabus

Week 1: Introduction to Enzymes
Lecture 1: Introduction Lecture 2: Classification and Nomenclature of enzymes (Part 1) Lecture 3: Classification and Nomenclature of enzymes (Part 2) Week 2:Structure of enzyme
Lecture 4: Enzyme Structure (Part 1) Lecture 5: Enzyme Structure (Part 2) Lecture 6: Enzyme Structure (Part 3) Week 3:Enzyme Production (Part 1)
Lecture 7: Cloning of Enzyme (Part 1) Lecture 8: Cloning of Enzyme (Part 2) Lecture 9: Over-expression in Host Week 4:Enzyme Production (Part 2)
Lecture 10: Extraction of enzyme Lecture 11: Purification Strategies (Part 1) Lecture 12: Purification Strategies (Part 2) Week 5:Enzyme Production (Part 3)
Lecture 13: Purification Strategies (Part 3) Lecture 14: Purification Strategies (Part 4) Lecture 15: Enzyme Characterization Approaches Week 6:Enzyme catalyzed Biochemical reactions
Lecture 16: Enzyme Catalyzed reactions (Part 1: Carbohydrate Metabolism) Lecture 17: Enzyme Catalyzed reactions (Part 2: Lipid and Protein Metabolism) Lecture 18: Enzyme Catalyzed reactions (Part 3: Detoxification) Week 7:Enzyme-Substrate interaction
Lecture 19:Enzyme-Substrate interaction (Part 1: Spectroscopic approaches) Lecture 20:Enzyme-Substrate interaction (Part 2: Isothermal Calorimetry) Lecture 21: Enzyme-Substrate interaction (Part 3: Surface plasma resonance) Week 8:Enzyme assay system and Kinetics
Lecture 22: Enzyme assay system Lecture 23: Enzyme Kinetics (Part 1) Lecture 24: Enzyme Kinetics (Part 2) Week 9:Enzyme Inhibitor Designing
Lecture 25: Inhibitor designing (Part 1: Traditional approach) Lecture 26: Inhibitor designing (Part 2: Modern approach) Lecture 27: Inhibitor designing (Part 3: Computational approaches) Week 10:Enzyme Inhibition kinetics
Lecture 28: Enzyme Inhibition kinetics (Part 1) Lecture 29:Enzyme Inhibition kinetics (Part 2) Lecture 30: Enzyme Inhibition kinetics (Part 3) Week 11:Enzyme Applications (Part 1)
Lecture 31: Enzymes in Industrial setup (Part 1) Lecture 32: Enzymes in Industrial setup (Part 2) Lecture 33: Enzymes in catalyzing chemical reactions Week 12:Enzyme Applications (Part 2)
Lecture 31: Enzymes in medical field Lecture 32: Enzymes in environment field Lecture 33: Enzymes in drug discovery