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Join a 46-minute webinar presented by Dr. Ling-Gang Wu, a senior investigator at NINDS, NIH, exploring groundbreaking insights into membrane transformation processes in live cells. Discover a newly developed workflow for real-time visualization of exocytosis and endocytosis in neuroendocrine chromaffin cells using advanced STED imaging techniques. Learn how membrane fusion and budding exceed classical understanding, involving complex transformations including hemi-to-full fusion, pore dynamics, and flat-to-Λ-to-Ω-shape transitions. Explore how various cellular mechanisms - including calcium/SNARE-dependent fusion machinery, cytoskeleton-dependent membrane tension, and actin/dynamin-dependent force machinery - work together to control release probability, synchronization, and content release. Gain deep insights into the molecular mechanics underlying these fundamental biological processes that are essential for intracellular trafficking, signal transduction, and membrane homeostasis. Master the visualization techniques and understanding of vesicle fusion and budding dynamics that may be conserved across various cellular processes.
