Explore the intricate world of chromatin organization and gene regulation in this conference talk delivered by Daniel Jost. Delve into the role of polymerases in shaping the four-dimensional genome structure. Learn about the latest advancements in understanding how these enzymes contribute to the spatial and temporal arrangement of genetic material within the nucleus. Gain insights into the complex interplay between DNA sequence, epigenetic states, and 3D chromatin organization in regulating gene expression. This talk is part of a comprehensive program on interdisciplinary aspects of chromatin organization and gene regulation, bringing together experts from various fields to discuss cutting-edge research and foster cross-disciplinary collaborations.