Feasibility on the Synthesis of Trimer Based DNA Sequences for Combinatorial DNA Encoding in Data

Explore the feasibility of combinatorial DNA encoding for data storage through this 22-minute conference presentation that demonstrates synthesizing DNA sequences using Bz-NPPOC and DMT phosphoramidites with trimer incorporation. Learn how researchers successfully integrated the "TAC" trimer into DNA sequences, achieving over 50% incorporation rates compared to control sequences using only monomers, while discovering that consecutive trimer presence decreases due to differential deprotection mechanisms between TCA-deprotected DMT phosphoramidites and UV-deprotected Bz-NPPOC phosphoramidites. Understand the challenges of incomplete deprotection and sequence loss caused by depurination during TCA exposure, and discover how Maskless Array Synthesis (MAS) technology overcomes these obstacles by using digital micromirror devices to spatially pattern UV light for precise, high-throughput synthesis of over two million sequences in parallel. Examine how MAS enables selective deprotection of Bz-NPPOC phosphoramidites at specific array locations, ensuring accurate DMT trimer coupling and creating numerous sequence combinations that significantly increase data storage density. Gain insights into developing scalable DNA storage solutions that combine monomers and trimers for creating large, high-density, error-resistant DNA libraries, setting the foundation for efficient DNA-based data storage systems presented by Hamed Sabzalipoor from the University of Munich at the 2025 Storage and Computing with DNA Conference.