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This 33-minute tutorial from DNA Learning Center guides educators through the complete process of working with MinKNOW software for Nanopore DNA sequencing, covering flow cell checking, sequencing initiation, experiment monitoring, and output file review. Follow along with detailed demonstrations at each stage: checking the MinION flow cell (00:00), starting the sequencing process (07:49), monitoring progress mid-run (17:22), and reviewing experimental results and output files (24:51). Designed specifically for educators introducing Nanopore technology in classroom settings, particularly for course-based undergraduate research experiences (CUREs), this video is part of a series explaining Nanopore sequencing fundamentals through demonstration experiments. Access additional educational resources at nanopore4edu.org.
