Characterization of Anti-HER2 Antibodies Using Advanced Flow Cytometry and BLI Label-Free Detection
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Overview
Syllabus
Intro
iQue® 3: Advanced Flow Cytometry Platform
Octet BLI Label-Free Detection: Dip and Read Biosensors
Types of Information Generated Using the Octet Platform
Biosensor Chemistries
Project Workflow
Antibody Binding Kinetics
Live-cell Competition Binding
Competition Between Anti-HER2 Antibodies
Relative Glycosylation of Anti-HER2 Antibodies
iQue® Antibody Internalization Kit
Measuring Cytotoxic ADC Payload Induced Killing
iQue® Human Antibody-Dependent Cellular Phagocytosis (ADCP) Kit
ADCP Kit Gating Strategy
Comparing NK ADCC Activity Between Anti-HER2 Antibodies
Wrap Up and Perspective
Taught by
Labroots