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Learn essential gene expression analysis techniques in this comprehensive tutorial video that guides through analyzing data post-GEO2R processing. Master the process of working with .csv files, filtering differentially expressed genes (DEGs), and performing functional annotations using Enrichr and ClueGO in Cytoscape. Explore step-by-step instructions for filtering DEGs based on Log Fold Change and P-values, conducting Gene Ontology (GO) annotations across biological processes, molecular functions, and cellular components, and performing pathway enrichment analysis. Discover how to utilize Enrichr for efficient web-based annotations and create visual representations of GO and pathway networks using ClueGO and CluePedia in Cytoscape. Perfect for beginners and those looking to enhance their bioinformatics skills, gain practical experience with essential tools including GEO2R, Enrichr, Cytoscape, ClueGO, and CluePedia while developing confidence in interpreting gene expression data for meaningful biological insights.
